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BROMCRESOL GREEN.

0000001428 00000 n Q5. 2471 0 obj <> endobj 0000010771 00000 n Find out more about how we use your information in our Privacy Policy and Cookie Policy.

Search results for bromocresol green at Sigma-Aldrich.

0000013138 00000 n You can change your choices at any time by visiting Your Privacy Controls. Bromocresol green (3,3',5,5'-tetrabromo-m-cresolsulfonphthalein) is a dye of the triphenylmethane family (triarylmethane dyes), which is used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis. The acid and basic forms of this dye have an isosbestic point in their UV-Visible spectrum, around 515 nm, indicate that the two forms interconvert directly without forming any other substance. 0000005250 00000 n The pKa of bromocresol green is given in the Merck Index. Skin and eye contact should be avoided. 0000007814 00000 n To enable Verizon Media and our partners to process your personal data select 'I agree', or select 'Manage settings' for more information and to manage your choices. 0

0000092640 00000 n 0000092368 00000 n <]>> 0000007046 00000 n An ethanol solution (0.04 wt%) of bromocresol green has been proposed for TLC staining and is suitable for visualisation of the compounds with functional groups whose pKa is below 5.0 (carboxylic acids, sulfonic acids, etc.). b) What is the literature value of the pKa for bromocresol green (Merck Index)?

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3,3-Bis(3,5-dibromo-4-hydroxy-2-methylphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide a) What relationship does the pKa bear to the Ka? In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue),[5] which is stabilized by resonance: The acid dissociation constant (pKa) of this reaction is 4.8. 0000000016 00000 n MDL number MFCD00005874. NACRES NA.47

0000004995 00000 n We and our partners will store and/or access information on your device through the use of cookies and similar technologies, to display personalised ads and content, for ad and content measurement, audience insights and product development. These appear as yellow spots on a light or dark blue background; no heating is necessary. 0000002660 00000 n

0000004456 00000 n 0000005499 00000 n The most common use of Bromocresol green is to measure serum albumin concentration within mammalian blood samples in possible cases of kidney failure and liver disease. Additional applications include use in sol-gel matrices,[8] the detection of ammonia,[9] and the measurement of albumin in human plasma and serum.[10]. 0000108158 00000 n In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue), which is stabilized by resonance: 0000007988 00000 n

0000012364 00000 n 0000003401 00000 n

2471 35 0000005734 00000 n
It belongs to a class of dyes called sulfonephthaleins. Precise pH measurement Absorption spectra of bromocresol green at different stages of protonation. *Please select more than one item to compare Buy Sigma Aldrich 114359-25G Bromocresol Green, ACS Reagent Grade, 25g Bottle %PDF-1.6 %âãÏÓ When bromocresol green is added to a solution, the pH is 2.88 (the color is yellow).

76-60-8. Bromocresol Green ACS reagent, Dye content 95 % CAS Number 76-60-8.
It is used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis. 0000002106 00000 n [6] Tap water is sufficiently basic to give a solution of bromocresol green its characteristic blue-green color.[7]. It can be used in its free acid form (light brown solid), or as a sodium salt (dark green … 0000006062 00000 n Empirical Formula (Hill Notation) C 21 H 14 Br 4 O 5 S .

It can be used in its free acid form (light brown solid), or as a sodium salt (dark green solid). 0000009967 00000 n Molecular Weight 698.01 . 2505 0 obj<>stream

bromo cresol green. 0000007950 00000 n 0000008355 00000 n

Q4. Bromocresol green may cause irritation. When it turns green, the pH is 3.88. 0000000996 00000 n Why can we determine the Ka of bromocresol green only semi-quantitatively? Calculate [bromocresol green], in mol/L, in the stock solution (0.04% (m/v)).

Bromocresol green. 0000083050 00000 n PubChem Substance ID 24847166. 0000045863 00000 n xref 0000009231 00000 n 0000001561 00000 n In order to determine the pKA of bromcresol green, a series of measurements must be performed at different pHs. Bromocresol green (BCG) is a dye of the triphenylmethane family (triarylmethane dyes).

[4] It is used as a pH indicator in applications such as growth mediums for microorganisms and titrations. 0000005812 00000 n Beilstein/REAXYS Number 372527 . 0000011461 00000 n

In clinical practise, it is commonly used as a diagnostic technique.

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0000015809 00000 n 2,6-Dibromo-4-[7-(3,5-dibromo-4-hydroxy-2-methyl-phenyl)-9,9-dioxo-8-oxa-9λ6-thiabicyclo[4.3.0]nona-1,3,5-trien-7-yl]-3-methyl-phenol, InChI=1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3, InChI=1/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3, Brc1cc(c(c(Br)c1O)C)C3(OS(=O)(=O)c2ccccc23)c4cc(Br)c(O)c(Br)c4C, Except where otherwise noted, data are given for materials in their, http://chemicalland21.com/specialtychem/finechem/BROMOCRESOL%20GREEN.htm, "BCG (Bromocresol Green) Albumin Assay Kit", https://en.wikipedia.org/w/index.php?title=Bromocresol_green&oldid=961946517, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, Soluble in benzene; very soluble in ethanol and diethyl ether, This page was last edited on 11 June 2020, at 07:31. Information about your device and internet connection, including your IP address, Browsing and search activity while using Verizon Media websites and apps. Compare Products: Select up to 4 products. When it turns blue, the pH is 5.52. The compound is synthesized by bromination of cresol purple (m-cresolsulfonphthalein). 0000092135 00000 n

It is also an inhibitor of the prostaglandin E2 transport protein.

startxref The pKA is equal to the pH at which the concentration of the two forms are equal. 0000001687 00000 n

Bromophenol blue solution can be used for the same purpose. EC Number 200-972-8. %%EOF 0000006319 00000 n

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